Raptor EtG/EtS Column > 컬럼

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Raptor EtG/EtS (cat.# 9325A12)

100 mm x 2.1 mm ID

2.7 um​

90 Å

UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)

35 °C

0.1% Formic acid in water

A 500 ng/mL QC sample was prepared in urine. 50 µL of the sample was diluted with 950 µL of a working internal standard (25 ng/mL EtS-d5/100 ng/mL EtG-d5 in 0.1% formic acid in water). The sample was vortexed at 3,500 rpm for 10 seconds to mix. The sample was then centrifuged at 3,000 rpm for 5 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.

10 µL

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time(min)   Flow(mL/min)   %A    %B

  0.00                 0.5            95       5

  2.50                 0.5            65      35

  2.01                 0.5            95       5

  4.00                 0.5            95       5

MS/MS

ESI-

MRM

HPLC

Reference Standards
Ethyl-β-D-glucuronide (cat.# 34101)

Ethyl-β-D-glucuronide-d5 (cat.# 34102)

Ethyl sulfate sodium salt (cat.# 34103)

Ethyl sulfate-d5 sodium salt (cat.# 34104)