ACE UltraCore™ SuperC18 > 크로마토그래피

Column: ACE UltraCore SuperC18, 50 x 2.1mm, 2.5μm Temp: 40°C Flow Rate: 0.40ml/min Detection: Agilent 6420 triple quadrupole MS, ESI +ve mode, dynamic MRM
Mobile Phase A: 0.1% v/v HCOOH + 5mM ammonium formate in 10:90 v/v MeOH/H2O Mobile Phase B: 0.1% v/v HCOOH + 5mM ammonium formate in 90:10 v/v MeOH/H2O
Gradient: Time (mins) 0 1  15   18 18.05 20
                              %B 0 0 100 100    0      0​

 Column: ACE UltraCore SuperC18, 100 x 2.1mm, 2.5μm Flow Rate: 0.50ml/min Temp: 45°C Detection: Waters Xevo TQS MS,
+ve or –ve mode as required, MRM data Mobile Phase A: 0.01mM NH4F + 0.001% v/v HCOOH (aq) Mobile Phase B: MeCN
Gradient: Time (mins) 0  0.5 7  7.5 10.5
                             %B 25 25 35  35   60

Additional –ve mode
(-ESI) analytes:

Taleranol and zeranol-d4
Taleranol and zeranol
Zearalenone
Hexestrol
Diethylstilbesterol
Dienestrol

Additional +ve mode
(+ESI) analytes:

Hydroxystanazolol
Hydroxystanazolol-d3
Methyltestosterone
Methyltestosterone-d3
β-Nortestosterone-d3
β-Trenbolone
α-Trenbolone

 Phase

 Functional Group

 Particle
Size (μm)

 Pore Size
(Å)

 Surface
Area (m2/g)

 Carbon
Load (%)

 Maximum
pH Range

 USPListing

 ACE UltraCore 2.5 SuperC18

 Octadecyl encapsulated

 2.5

 95

 130

 7.0

 1.5-11.0 (*)

 L1

 ACE UltraCore 5 SuperC18

 Octadecyl encapsulated

 5

 95

 100

 5.4

 1.5-11.0 (*)

L1